Yeast Nitrogen Base without Amino acids, without Ammonium sulphate and without para Amino Benzoic acid

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Description

Yeast Nitrogen Base without Amino acids, without Ammonium sulphate and without para Amino Benzoic acidPara Amino benzoic acid is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of para Amino Benzoic acid or to replace this vitamin. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water

13 pH (100 g/l) 5

MB containing Dextrose

Suspend 31

Both Folic acid and Riboflavin are omitted from the medium to make it suitable for fluorescence measurements with yeast cultures

Vegetable Peptone 10 g/L Yeast extract 7 g/L Potassium Dihydrogen Phosphate 1

16 pH (100 g/l) 5

4 (100 g/l) Assay ≥99%

49 Na2HPO4

The alterations in amino acid formulation of SIH medium resulted in a more even and better amino acid utilisation

Formulation

Tryptone 10 Yeast extract 5 NaCl 0

such as lactic acid